It's not that critical, replenish about every 7 enemas/flushes. If you eat plenty of good veggies they should be able to replenish on their own. :) BUT megadosing on probiotics is one way to get over candida faster, so do see about getting a good one. Many people like PRimal Defense, but you can't implant that via the enema, you have to swallow it. I find it very effective to get the live acidophilus culture from the refrigerated section and add that to the enema solution. Or have my colonics person implant some after a colonic.