Re: Meat into Vinegar experiment revisited

Hello, Rabbitears.


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>> Should kill most according to what I've read, IF it's done properly.
I have frozen specimens at -10 Celsius for two hours and MOST OF THE TIME I haven't found further signs of any biological activity in them, probably meaning they 'died'.<<

Many years ago I read that upon closer examination of frozen meat, parasites are still alive in a "frozen" stasis.
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That's what I'm always looking out for.




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When thawed out, they can move but much slower. I'm curious to know, assuming that you allowed the meat to thaw, how much time elapsed before concluding that they were dead?
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First of all, I never concluded that they were 'dead'. I shouldn't, because there are a great number of variables involved, and sometimes you just can't say that a specimen is dead simply because it doesn't move. What I have recorded is "no signs of biological activity" for a given length of time. I have recorded 7 days length as a max, if I remember correctly (never less than 48hrs). However, those 7 days are NOT an absolute guarantee of 'death' either, because there are certain specimens that took 3 weeks or more to show activity, so a valid argument could be "no observable biological activity" for a given period of time. Those margins were acceptable for me because I was just after results. My reasoning was that in 7 days I should have at least one bowel movement, right? So that should be more than enough time for me to get rid of the bug through natural digestion-expulsion, or so I hoped.

It all makes you reconsider 'death', in a way. I have a specimen in storage that I carefully check from time to time, and after over two years of storage it is still biologically active. Shouldn't that tell us something?







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I think this is fantastic that we have an oppty to ask these type of questions and get them answered with someone who is interested in these subjects and doing experiments.

Many thanks to the above posters who gave detailed descriptions to what you viewed. This is very helpful to some of us who can't view pics, at the moment.

Thanks!
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I'm glad if I can help a bit.
One thing I learned first is that the world of microscopy is awe-inspiring, and the images can be confusing and often deceiving, but there is an 'art' in it that eventually helps us to set a difference between "something" and "probably nothing". I hope that all of us can share that art.

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