The microvilli of the follicular cells produces an enzyme called human thyroid peroxidase. This enzyme oxidises Iodide molecules trapped in the colloid into Iodine (the active form of the Iodide molecule). This reaction produces peroxide radicals which must be neutralized by antioxidants. The Iodine then combines with the amino acid Tyrosine to form mono-iodotyrosine or T1. Then two of these molecules combine to form di-iodotyrosine, T2. The peroxidase enzyme then couples a T1 to T2 to form T3 or combines two molecules of T2 to from T4. T3 and T4 are the thyroid hormones with biological activity. http://www.drwells.net/nutrition/topics/Hypothyroid.htm