Apparently the cold brewed method will last for 14 days in the fridge and has 67% less acid than a hot brew. Personally i use 1 part coffee concentrate with 3 parts water and will only use organic coffee. Coffee enemas done right are held for fifteen to twenty minutes. This causes the liver and gall bladder to secrete bile that eliminate the toxins in the body. http://www.iamnatural.com.au/seven-reasons-to-try-a-coffee-enema/