Beginning new quest for plasmids.

I have started a culture from stool specimen, by streaking nutrient plate. I have the e coli colonies already. I plan to purify the e coli until I narrow down the high repeats of foreign bacteria. I will try and use some innovative methods to create protein profiles of high purity selectives.
From there, I will try cross-transfection with yeast cells to see if there is unsusual phenomenon present.
I am pondering if scalp parasites are systemic, and involve commensual yeast in anyway.
I would also like to take notice of any strange mutations of yeast, because they are a primal representation of eukaryotic cells. I should check them out as well for plasmids. Shroom Tweet