Hitt it multiple times a day( A shot every hour or 2 for a couple of days) My grandson used the nebulizer every 2 hours and did it 8 times a day. By the next day he was doing so much better. By the end of the 2nd day he was jumping around and did not look or act like he was sick and when we asked he said:" all better now!" He went to preschool the next day and was the only one to show up! It took another 4 days before anyone else showed up. Several of the other parents at the (church/school) asked what we were doing as they noticed that Parker when sick(not often!) was not sick for long and they wanted to know how we were doing this! Several knew about silver but did not realize hoiw well it worked!