Make sure the water is as cold as you can get it without freezing, the colder water holds more ozone than room temp. I let my 400mg/hr ozone generator run for about 45 minutes into cold water(almost frozen) then drink it within 30 minutes. The big thing is doing all of the protocols daily and doing CS and ozone water several times a day. My friend has diabetes and is very large(380lbs) she had a very bad strip of open wound area from the knee to her ankle. Using CS(20ppm I made) and then about 2 hours after the CS has dried I would pour the ozone water over the area, as well she was taking CS and ozone water taken orally. In less than 3 weeks the area healed! Her doctor could not believe it! she had been given everything to heal the area up and had not worked.