VIRIAL DISEASES DISABLED BY 50 TO 100 MICROAMPRES OF CURRENT

Paper by W. Lyman, et a1. Reporting Inactivation of AIDS Virus by Electric Curr
or
http://www.papimi.gr/safe-hiv/AppendixE.htm
http://groups.yahoo.com/group/microelectricitygermkiller/

Dear Sir/Madam.
i write to inform you that the treatment for Aids was actually discovered 10 years ago and involved passing a small electrical current of 100 microamperes through the infected blood and this disabled the aids virus.
As this method did not involve the use of drugs the pharmaceutical companies who are very powerful managed to suppress any attempts to bring this to
mainstream usage.As a result of this the original research paper only appeared in 3 publications and has not been allowed to be mentioned again.

A man called Robert Beck actually implemented the cure using electrodes attached to the wrist and passing a small current through them.you can find out more by typing Robert Beck in web browser or 'blood electrification'.

The pharmaceutical companies would lose a lot of money if the method was used on a large scale and they are maintaining their drive to stop manufacture of the beck device by shutting down jaguer enterprises last week as they manufactured these devices.
Your position allows you to bring this information to a wider audience and i ask you to study this for yourself and help the many people who are suffering from Aids , Cancer , Malaria and Typhoid both in the U.k. and in third world countries as well.
I understand the incidence is 1 in 3 now for cancer now.

Last year 126000 people died fom cancer in the u.k.
last night i heard the playwright Harold Pinter had it.
George Harrisons £140 million fortune did not save him as the doctors did not tell him and used the most advanced chemotherapy instead.Remember cancers are caused by bacteria and viruses.

The method can also be applied to cancer causing viruses.Remember the passing of a small electrical current of between 50 to 100 microamperes disables the virus and the very low cost of such a method has resulted in its suppresssion as the drug companies would lose billions as their expensive drugs are sidestepped.
the device can be made easily by using four 9 volts batteries in series and a variable resistor to adjust the current plus wet cloth covered 1 cm square electrodes taped to each of the pulse points on each wrist.
you can find more detailed information if you type blood electrication in your web browser.
i am not selling you anything.
the research paper is detailed below and is available on the internet at:-
http://www.papimi.gr/safe-hiv/AppendixE.htm

yours sincerely.
dr callum
Positive electricity experiments on HIV-1 virus.Lab Test
Results of HIV inactivation by electric current from patent 5,139,684 (of
Kaali & Schwolsky 8-18-92)

EXPERIMENTAL RESULTS

Overview: A non-flow vessel or cell included a pair of platinum electrodes
1 mm apart inserted into a well 1.56 mm in length and 8.32 mm in depth.
The non-flow vessel was connected to a direct current source capable of
creating an electric field at a constant voltage and constant amperage.
Into this well was laced a suspension of the human immunodeficiency virus
type 1 (HIV-1) at a concentration of 1,000,000 infectious particles per
ml. An aliquot of approximately 10 ul of the virus suspension was placed
into the well. Thereafter, the viral suspension was exposed to direct
currents ranging from 0 microamps (uA) for up to 12 minutes, to 100
microamps for up to 6 minutes. Intermediate currents of 25, 50 and 75
microamps were used to expose similar viral aliquots. After exposure of
the viral suspension to electric currents, the contents of the non-flow
vessel were removed and placed into sterile microtubes. 5 ul of each
sample were removed and diluted with 95 ul tissue culture medium
supplemented with 10% fetal calf serum (FCS. unborn calf blood)
In Experiment 1, the resuspended and treated viral stocks were incubated
with a human T lymphoblastoid cell line named CEM-SS. This cell line, upon
exposure to HIV-1, forms syncytia (giant cells). It is well documented
that the viral titer (amount) used is directly correlated with the number
of syncytia formed. Therefore, evaluation of infectivity of HIV-1 can be
used with this assay. In contrast, Experiment No. 2 used a differnet human
T lymphoblastoid cell line named H9. This cell line, in contrast to CEM-SS
cells, produces, upon exposure to HIV-1, many viral particles. The amount
of virus produced is proportional to the amount of virus to which the
cells are exposed. Therefore, quantitation of viral particles, or more
commonly associated viral protein (in this case reverse transcriptase),
can be used as an index of viral infection. In both assays, the CEM
syncytia forming assay and the H9 viral protein assay, similar type
results were obtained. That is, with the CEM cells, although syncytium
formation and quantitation is preferrable, one can quantitate the HIV-1
associated protein (reverse transcriptase) activity and conversely with
the H9 cells, although reverse transcriptase quantitation is preferred,
one can quantitate giant cell (syncytia) formation. Both of these assays
are widely used as reproducible measures of viral infection and can be
used to determine if alterations in viral infectivity as a product of this
electrical treatment can be detected.

Experiment #1

Approximately 100,000 CEM-SS cells per sample were incubated with a
treated or untreated (control) viral aliquot for up to 4 days. The cells
were placed into microtiter plate wells and monitored for formation of
syncytia every 24 hours by microscopic observation. In a standardized
fashion, as it has been reported in the literature and is currently being
conducted in many laboratories, the number of syncytia at 3 and 4 days was
determined. Table 2 summarizes the results from a representative
experiment using this assay. As can be noted, the number of syncytia
formed was inversely proportional to the amount of electric current. That
is, additionally, with increased current (100 vs 50 uA) there was a
reduction in the number of syncytia formed. These results and those of
additional experiments using the CEM-SS cell line indicate a consistent
finding that electrical treatment of the RF strain of HIV-1 attentuates
the virus potential for inducing syncytium formation in this cell line.


Experiment #2
A separate and independent assay to determine the ability of electric
current to alter HIV-1 infectivity using H9 cells was employed. The basic
strategy was similar to that used for the CEM cells with the exception
that the initial suspension of treated and controlled (non-treated) viral
stock was incubated with 100,000 H9 cells for 2 hours at 37 degees
Celsius. Thereafter, the cell virus suspensions were further diluted to 5
ml in standard tissue culture medium. The cell-viral suspensions were then
incubated for up to 14 days at 37 degrees Celsius with 5% carbon dioxide.
At 3 day intervals (beginning at day 2), aliquots of cell suspension were
removed from each sample. The aliquots were centrifuged at 1,000 rpm for 5
minutes in order to pellet the cells. After centrifugation, the
supernatant and cell pellets were seperated. The supernatant was
cyropreserved for subsequent reverse transcriptase assay and the cell
pellets were resuspended in fixatives and maintained in a tissue bank for
additional studies employing in situ hybridization and immunocytochemistry
to detect qualitatively and semi-qualitatively viral infection by HIV-1.
At the end of each experiment, the supernatant samples from each of the
tests and time points were examined using standard reverse transcriptase
assay. The results of the representative experiment are shown in Table 3.
The results of this experiment indicate the ability of HIV-1 to infect H9
cells is attenuated by the magnitude of the electrical currents to which
the virus is exposed. Additionally, at lower current magnitude, but with
prolonged exposure time, attenuation of viral infectivity is achieved.
That is, analogous to the results observed using syncytium formation and
the CEM-SS cell line, either increased current or increased duration of
exposure time was inversely proportional to the amount of reverse
transcriptase produced by the cell line.

In conclusion, these experiments which have been repeated several times,
and those using the CEM-SS cell line, indicate at a statistically
significant level that direct electrical current at biocompatible
amperages for discrete exposure time intervals can attenuate the ability
of HIV-1 to infect normally healthy cells which are susceptible to the
HIV-1 AIDS virus.



TABLE 2
Syncytium Formation
------------------------------------------
Dilution
of virus (Number of Syncytia)
-------- --------------------------------
1:20 TNTC TNTC 28 66 15
1:40 TNTC 175 22 42 7
1:80 TNTC 90 20 25 4
1:160 180 44 9 9 2
1:320 115 28 4 6 0
1:640 70 10 0 2 0
1:1280 40 7 0 0 0
1:2560 28 4 0 0 0
1:5120 15 2 0 0 0
1:10,240 10 1 0 0 0
1:20,480 4 0 0 0 0
------ ----- ----- ----- ------
0uA 25uA 50uA 75uA 100uA
---------------------------------------------
(TNTC=too numerous to count)



TABLE 3
Reverse Transcriptase Activity
(count per million x .001)
-------------------------------------
Days of Incubation
------------------
uAmps/Time(min.) 2 days 4 days
---------------- ------- ------
0/6 0 13.8
0/12 0 11.7
50/3 0 9.1
50/6 0 9.1
50/12 0 4.8
100/3 0 5.7
100/6 0 3.6
------------------------------------






Click here to read about an electro-medical device that puts electricity
into the blood for the inactivation (decreased infectivity) of HIV and
other microbes


Lab Test Results of HIV inactivation by electric current from patent 5,139,684 (of Kaali & Schwolsky 8-18-92)

--------------------------------------------------------------------------------


EXPERIMENTAL RESULTS

Overview: A non-flow vessel or cell included a pair of platinum electrodes 1 mm apart inserted into a well 1.56 mm in length and 8.32 mm in depth. The non-flow vessel was connected to a direct current source capable of creating an electric field at a constant voltage and constant amperage. Into this well was laced a suspension of the human immunodeficiency virus type 1 (HIV-1) at a concentration of 1,000,000 infectious particles per ml. An aliquot of approximately 10 ul of the virus suspension was placed into the well. Thereafter, the viral suspension was exposed to direct currents ranging from 0 microamps (uA) for up to 12 minutes, to 100 microamps for up to 6 minutes. Intermediate currents of 25, 50 and 75 microamps were used to expose similar viral aliquots. After exposure of the viral suspension to electric currents, the contents of the non-flow vessel were removed and placed into sterile microtubes. 5 ul of each sample were removed and diluted with 95 ul tissue culture medium supplemented with 10% fetal calf serum (FCS. unborn calf blood)
In Experiment 1, the resuspended and treated viral stocks were incubated with a human T lymphoblastoid cell line named CEM-SS. This cell line, upon exposure to HIV-1, forms syncytia (giant cells). It is well documented that the viral titer (amount) used is directly correlated with the number of syncytia formed. Therefore, evaluation of infectivity of HIV-1 can be used with this assay. In contrast, Experiment No. 2 used a differnet human T lymphoblastoid cell line named H9. This cell line, in contrast to CEM-SS cells, produces, upon exposure to HIV-1, many viral particles. The amount of virus produced is proportional to the amount of virus to which the cells are exposed. Therefore, quantitation of viral particles, or more commonly associated viral protein (in this case reverse transcriptase), can be used as an index of viral infection. In both assays, the CEM syncytia forming assay and the H9 viral protein assay, similar type results were obtained. That is, with the CEM cells, although syncytium formation and quantitation is preferrable, one can quantitate the HIV-1 associated protein (reverse transcriptase) activity and conversely with the H9 cells, although reverse transcriptase quantitation is preferred, one can quantitate giant cell (syncytia) formation. Both of these assays are widely used as reproducible measures of viral infection and can be used to determine if alterations in viral infectivity as a product of this electrical treatment can be detected.

Experiment #1

Approximately 100,000 CEM-SS cells per sample were incubated with a treated or untreated (control) viral aliquot for up to 4 days. The cells were placed into microtiter plate wells and monitored for formation of syncytia every 24 hours by microscopic observation. In a standardized fashion, as it has been reported in the literature and is currently being conducted in many laboratories, the number of syncytia at 3 and 4 days was determined. Table 2 summarizes the results from a representative experiment using this assay. As can be noted, the number of syncytia formed was inversely proportional to the amount of electric current. That is, additionally, with increased current (100 vs 50 uA) there was a reduction in the number of syncytia formed. These results and those of additional experiments using the CEM-SS cell line indicate a consistent finding that electrical treatment of the RF strain of HIV-1 attentuates the virus potential for inducing syncytium formation in this cell line.


Experiment #2
A separate and independent assay to determine the ability of electric current to alter HIV-1 infectivity using H9 cells was employed. The basic strategy was similar to that used for the CEM cells with the exception that the initial suspension of treated and controlled (non-treated) viral stock was incubated with 100,000 H9 cells for 2 hours at 37 degees Celsius. Thereafter, the cell virus suspensions were further diluted to 5 ml in standard tissue culture medium. The cell-viral suspensions were then incubated for up to 14 days at 37 degrees Celsius with 5% carbon dioxide. At 3 day intervals (beginning at day 2), aliquots of cell suspension were removed from each sample. The aliquots were centrifuged at 1,000 rpm for 5 minutes in order to pellet the cells. After centrifugation, the supernatant and cell pellets were seperated. The supernatant was cyropreserved for subsequent reverse transcriptase assay and the cell pellets were resuspended in fixatives and maintained in a tissue bank for additional studies employing in situ hybridization and immunocytochemistry to detect qualitatively and semi-qualitatively viral infection by HIV-1. At the end of each experiment, the supernatant samples from each of the tests and time points were examined using standard reverse transcriptase assay. The results of the representative experiment are shown in Table 3. The results of this experiment indicate the ability of HIV-1 to infect H9 cells is attenuated by the magnitude of the electrical currents to which the virus is exposed. Additionally, at lower current magnitude, but with prolonged exposure time, attenuation of viral infectivity is achieved. That is, analogous to the results observed using syncytium formation and the CEM-SS cell line, either increased current or increased duration of exposure time was inversely proportional to the amount of reverse transcriptase produced by the cell line.

In conclusion, these experiments which have been repeated several times, and those using the CEM-SS cell line, indicate at a statistically significant level that direct electrical current at biocompatible amperages for discrete exposure time intervals can attenuate the ability of HIV-1 to infect normally healthy cells which are susceptible to the HIV-1 AIDS virus.

TABLE 2
Syncytium Formation
------------------------------------------
Dilution
of virus (Number of Syncytia)
-------- --------------------------------
1:20 TNTC TNTC 28 66 15
1:40 TNTC 175 22 42 7
1:80 TNTC 90 20 25 4
1:160 180 44 9 9 2
1:320 115 28 4 6 0
1:640 70 10 0 2 0
1:1280 40 7 0 0 0
1:2560 28 4 0 0 0
1:5120 15 2 0 0 0
1:10,240 10 1 0 0 0
1:20,480 4 0 0 0 0
------ ----- ----- ----- ------
0uA 25uA 50uA 75uA 100uA
---------------------------------------------
(TNTC=too numerous to count)

TABLE 3
Reverse Transcriptase Activity
(count per million x .001)
-------------------------------------
Days of Incubation
------------------
uAmps/Time(min.) 2 days 4 days
---------------- ------- ------
0/6 0 13.8
0/12 0 11.7
50/3 0 9.1
50/6 0 9.1
50/12 0 4.8
100/3 0 5.7
100/6 0 3.6
------------------------------------

Dr Robert Beck implemented the above using wrist or arterial electrodes put on the surface of the skin over major blood arteries and passing the current through the skin.You can find more information by typing blood electrification on your web browser.

i hope to hear a response from you soon
yours sincerely ,
dr callum



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