Do at least one regular flushing enema first just to get the dreck out, then do an ACV enema. Basically anytime you add something to an enema solution, start with about 1/2 cup to about one quart of water for a retention enema, then adjust as necessary. Try to retain for 5-10 minutes. The ACV enema is great for taking out candida and balancing the pH of the intestines themselves, which eventually makes it easier for friendly microbes to propagate.