In order to probe the activity of G6PDH, I tried to feed the Breast Cancer cell line with 13C-labeled glucose, and then isolated DNA. I further degrade the DNA to get deoxyribose following the procedure http://www.e-biotek.com/ebiotek_073.htm
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I wonder how I can purify or clean up the mixture with deoxyribose for GC/MS analyses.
Any advice is welcome. Thank you for your time in advance.