EXAMPLE 4

A test was designed to determine the effect on the growth in vitro of Candida albicans of xylitol, lactitol, sorbitol and aspartame in comparison to glucose and a non-carbohydrate control medium. The tested substances have all been used in the food industry as sweeteners. The sweetness of the substances in the above order is respectively 100%, 40%, 60% and 20.000% of the sweetness of glucose.

12 strains of Candida albicans obtained from patients with AIDS and oral candida mucositis were evaluated. The strains were inoculated on Sabouraud agar plates (20% dextrose) and incubated at room temperature for 48 hours. One colony from each plate was inoculated in Muller Hinton broth (without sweetener) and left for 18 hours at 35° C. The tubes were standardized to Mac Farland 0.5 (5×10⁶ CFU/ml) and 100 μl inoculated in triplicate in spectrophotometer tubes containing 3 ml of Muller Hinton broth at final concentrations of glucose (5% and 10%); xylitol (5% and 10%); lactitol (5%, 8.3% and 10%); sorbitol (5%, 10% and 12.5%); and aspartame (0.025% and 0.05%). 3 tubes with Muller Hinton broth without carbohydrate was also inoculated to serve as controls.

Optical densities (650 nm) were determined at 0, 24 and 48 hours time. The average of three readings per time, per sweetener concentration was calculated and recorded. The results of the test are indicated in FIG. 2 showing the summary curve of the averages of all the strains in each of the test mediums.

The results clearly indicate that glucose has an effect of increasing the growth of Candida in comparison to the control without carbohydrate. The artificial sweeteners all reduced the growth of Candida compared to the control. Xylitol at 10% concentration proved to be by far the best of the tested compounds at reducing the growth of Candida in vitro.

* * * * *

the last paragraph states reduced growth compared to control culture. seems amazing to me. but weird it worked better in actual people using it along with the prescription anti-fungals, would love to see some more tests with  different variables, more people and using natural antifungals.