Very cool experiment Tony, but some things I think you might consider if you're going to continue experimenting.
Thanks, and thanks for your comments.
1) Isn't S.Boulardii a good yeast/probiotic? Also this test only proves that in the environment with only honey(food) and S.Boulardii, Vit C kills S. Boulardii
True, S. Boulardi is not pathogenic. The purpose of the test was to clear up some confusion regarding whether Vitamin C is cidal for yeasts. There is conflicting information on the subject.
Your second point is also valid. It's admittedly, a simple in vitro experiment and I agree that you have to be careful what conclusions you draw.
2) Wouldn't it be more beneficial to do this on fungus/yeast that is pathogenic?
Sure it would. But I don't have access.
3) What about repeating the test for other probiotics to see what good bacteria it kills? (Although I think this is less pertinent since the SB is supposed to be pretty sterile ideally right?)
Yes, those would be good experiments, particularly with something like B. Subtilis. Yes again. The context of the experiment is the question of how effective Vitamin C is at sterilizing the small bowel.
T.
True, S. Boulardi is not pathogenic. The purpose of the test was to clear up some confusion regarding whether Vitamin C is cidal for yeasts. There is conflicting information on the subject.
But this does not clear up anything. Vitamin C is an acid (ascorbic acid). But when acids leave the stomach they are neutralized by bicarbonate. Even if you took enough to overwhelm the bicarboanate buffer system this will just strain the body's buffer system leading to other health issues. In addition the vitamin C will still come in to contact with other substances in the intestines leading to further neutralization or binding of the ascorbic acid not already being absorbed. For example synthetic ascorbic acid is extremely unstable and can be broken down in to toxic oxalic acid. This can either be absorbed leading to the possibility of kidney stones or it bind to minerals in the intestines forming insoluble oxalates. These are good examples why "test tube" studies do not tell us how something will function in the body.
What happens in a jar is not necessarily the same as what happens in the body. Vitamin C also kills semen on contact, but this does not mean taking vitamin C orally is a means of birth control.