The gallbladder and the liver is subject to a cycle which occurs after eating and another cycle that occurs depending upon the time of day. In the evening between the hours of 10.00pm and 02.00am the blood flow is increased to the liver and gallbladder and during this time the liver replenishes the bile and at the same time it is concentrated in the gallbladder. During the evening after 18.00 hrs the gallbladder expands the greatest after contraction and in the morning around 09.00am it contracts the most. Both contraction and expansion are required for liver-flushing, which is achieved by 2 application of Epsom Salts and one of the oil potion. In practice the only time of day that gives this 6 hour window is between 18.00hrs and 2.00 am. In the morning by 12.00 midday the gallbladder is least animated. The other reason for nighttime flushing include safety because it is better to be in bed for those who get unsteady with dehydration.

Bile in terms of volume is replenished quickly but the components parts take different times and the bile salts around 12 days. Bile is added to by bicarbonate and water in the hepatic ducts during a Liver Flush and this overall flow is just as important as is the volume of bile. If the quantity of bile in the gallbladder is low the liver will attempt to compensate.

Which protocol is best whether when doubled up is a matter of opinion and dependent upon the type of biliary condition. For most people a regular protocol is sufficient.